Motorola LS6000B-U Betriebsanweisung Seite 129
- Seite / 180
- Inhaltsverzeichnis
- LESEZEICHEN
Bewertet. / 5. Basierend auf Kundenbewertungen
LS 6000 Scintillation System User’s Manual
PN 247971-F
7-3
How Sample Preparation Affects Results
Recognizing And Avoiding Statics
7
If you are unable to eliminate the source of single photon events, Lum-Ex Correction (if
installed) can subtract all contributions from single events from each isotope window.
7.2 Recognizing And Avoiding Statics
Statics are a common source of erratic high counts, resulting from a static charge on the
surface of the sample vial discharging in the counting chamber.
Static charges accumulate on sample vials when the vials are handled during sample
preparation. The instrument is equipped with an electronic ion source that will neutralize
static charges under most circumstances.
Certain conditions, however, may cause such large static build-ups that an appreciable charge
remains on the vial. The problem is most prominent in hot, dry weather or in the winter
when the indoor air is heated and dry. However, statics can also be seen in rainy, damp
weather. The use of plastic vials, especially when handled while wearing latex gloves, is
another major source of static build-up.
To check for the presence of statics, count one of your own samples (not an unquenched
standard) repeatedly over a period of time. If the count rate is erratic (increasing and
decreasing cpm) the presence of statics is likely.
The following steps should be taken when a statics problem is encountered:
1. Use vinyl or polyethylene gloves, not latex.
2. Treat the gloves with anti-static spray and/or wipe them with an anti-static cloth.
Treatment with an anti-static type fabric softener cloth, such as the type commonly used
with a house-hold clothes dryer, is particularly useful since it can prevent statics for
many days.
3. Wipe each vial completely with the anti-static cloth. Statics is very localized on a vial so
the entire vial must be wiped-sides, top and bottom.
Figure 7.1 shows how the effect of statics and how wiping a vial can immediately reduce
statics to normal background.
7.3 Two Phase Samples
A two-phase sample—that is, one that has separated into two distinct phases before or during
counting—may yield variable and highly inaccurate data from the counting process. In
addition, accurate quench monitoring is not possible. A sample may be thoroughly emulsified
when placed in the instrument, but may separate while waiting to be counted. Although the
two phases are sometimes visually distinct, in other cases the only difference that can be seen
is a slight haze, noticeable only on close observation. When plastic vials are used, even a
distinct separation would, of course, not be visible.
Samples separate into two phases because the sample exceeds the ability of the emulsifier to
make all of the aqueous samples soluble with the organic solvent of the cocktail. This
overload could be from too much sample, high salt concentrations, or extreme pH’s which
degrade the emulsifier. If there is a change in room temperature (sometimes only a few
degrees), a single phase sample can become two phase.
- LS 6000 Series 1
- Table of Contents 3
- Introduction 11
- Principles of Operation 12
- Operating the Instrument 12
- Operating Modes 13
- 1.3 Specifications 14
- Precautions and Limitations 15
- Figure 1.1 Standard Vial 15
- Miniature Vials 16
- 1.5 Hazards 17
- Maintenance 18
- Getting Started 19
- 2.2 Power Failure 21
- 2.3 Operating Controls 21
- Operator Control Key pad 22
- Using the Operating Controls 24
- 2.4 Using the Racks 25
- Using the Racks 26
- Setting Up the Racks 27
- 2.5 Preparing the Printer 29
- 2.6 System Setup 30
- Energy Scale 31
- Printer Paging 32
- Allow Interrupt Count 32
- System Setup 33
- Figure 2.9 RS232 Setup Menu 33
- Date/Time 35
- Auto DPM 35
- 2.7 Calibration 36
- Counting Samples 39
- Starting the Automatic Count 40
- Conducting Count Single Rack 41
- 3.4 Auto DPM 46
- 3.5 Multi-Task 47
- Interrupt Count in Multi-Task 48
- Multi-Task 50
- Interrupt Data 51
- Setting Up User Programs 59
- 4.2 Editing A User Program 61
- Editing A User Program 62
- Comments 65
- Counting Time 65
- Liquid or Xtal Scintillator 65
- Isotope 1 65
- Isotope 2 and Isotope 3 66
- Edit Other Parameters 67
- Counting Precision 69
- Background/Blank Subtraction 69
- Lum-Ex Correction 71
- Phase Monitor 72
- Low Level 72
- Low Count Reject 72
- Output Formats 72
- Count Data 75
- Raw Data 75
- Collecting Spectral Data 76
- 4.3 Copy User Program 77
- Copy User Program 78
- 5.2 CPM/Xtal CPM 80
- CPM/Xtal CPM 81
- Results for CPM/Xtal CPM 83
- 5.4 Auto DPM 85
- Setting Up Auto DPM 86
- Loading Samples for Auto DPM 86
- Results for Auto DPM 86
- Setting Up DPM 87
- 5.6 Xtal DPM 89
- Xtal DPM 90
- Setting Up Xtal DPM 91
- Loading Samples for Xtal DPM 92
- 5.7 DPM % of Reference 93
- Setting Up DPM % of Reference 94
- Xtal SL DPM % of Reference 95
- 5.9 Single Photon Monitoring 98
- Data Calculation 100
- Single Photon Monitoring 100
- Isotope/DPM Libraries 103
- Accessing the Isotope Library 104
- Printing the Isotope Library 104
- The Isotope Library 105
- Setup Menu 106
- PN 247971-F 108
- 5 minutes 109
- 6.2 Setting Up A Quench Curve 110
- Setting Up A Quench Curve 112
- Counting the Standards 114
- Editing Quench Curves 120
- Deleting A Quench Curve 124
- Manual Entry of Quench Curves 125
- 7.1 Chemiluminescence 127
- Interpretation of Results 128
- Reducing Single Photon Events 128
- 7.3 Two Phase Samples 129
- Procedures 131
- Analysis of Data 131
- H Counting Efficiency 132
- C Counting Efficiency 132
- Background 133
- Contamination 133
- Instrument Specifications 135
- Temperature Control Accessory 138
- Installation Requirements 139
- Electrical Requirements 140
- 0.5 to 1.0 m in 140
- Environment Requirements 142
- . Note that the output 144
- Datum IDs 146
- CHAPTER 1, PART 31 167
- Region I 170
- Region II 170
- Region III 170
- Region IV 170
Verwandte Produkte und Handbücher für Barcodeleser Motorola LS6000B-U
Barcodeleser Motorola SYMBOL MS320X Spezifikationen
(428 Seiten)
(428 Seiten)
Barcodeleser Motorola SB1 Betriebsanweisung
(82 Seiten)
(82 Seiten)
Barcodeleser Motorola MC17T Bedienungsanleitung
(24 Seiten)
(24 Seiten)
(32 Seiten)
Barcodeleser Motorola MP6000 Spezifikationen
(200 Seiten)
(200 Seiten)
Barcodeleser Motorola DS9208-1D Spezifikationen
(380 Seiten)
(380 Seiten)
Barcodeleser Motorola SYMBOL LS7708 Spezifikationen
(380 Seiten)
(380 Seiten)
Barcodeleser Motorola WT4090 Spezifikationen
(206 Seiten)
(206 Seiten)
(2 Seiten)
Barcodeleser Motorola LS1000B Spezifikationen
(96 Seiten)
(96 Seiten)
© 2020, manymanuals.de. Alle Rechte vorbehalten. | 0.038 s |
Manymanuals.com
Manymanuals.de
Manymanuals.fr
Manymanuals.it
Manymanuals.pl
Manymanuals.cz
Manymanuals.es
Manymanuals-pt.com
Kommentare zu diesen Handbüchern