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LS 6000 Scintillation System User’s Manual
PN 247971-F
1-1
Introduction
Intended Use
1
1
Introduction
1.1 Intended Use
The Beckman LS 6720/6730/6750 Scintillation System is designed to provide highly accurate,
automated counting of the level of radioactivity in radioactively-tagged samples. The
instrument can perform several types of calculations on the data obtained from counting, as
selected by the user.
1.2 General Description
Description of the Instrument
The Beckman LS 6720/6730/6750 Scintillatin System is a state-of-the-art bench top liquid
scintillatin counter, featuring a Motorola 68000 Series microprocessor, a Digital Signal
Processor and a 32,768 channel Multichannel Analyzer.
The instrument is available with either a monochrome or a color monitor; Hot Graph is
included with the color monitor system. The monitor displays an extensive user-interface
system that includes Help screens and instructions. The robust rack sample changer will
accommodate either 336 standard vials or 648 miniature vials (depending upon the version.)
Basic System
The system calculates counts per minute (cpm). The standard quench monitor is the IC#.
Data can be normalized and blanks and/or backgrounds can be subtracted. Percent of
reference can be calculated. Other standard features include a high count rate terminator that
automatically rejects samples with too much activity, an electrostatic controller to reduce the
interference of static electricity, and an 80-column printer for output of data. Twenty
different User Programs, which are the instructions for processing either a single sample or a
batch of samples, can be stored. Different racks of samples can be counted under different
User Programs without user intervention. An Isotope Library stores the window settings for
access by any User Program. Automatic operation can be interrupted to allow processing of
priority samples or to edit a User Program.
Value System
The Value System includes all the features of the Basic System, plus Versa-Rack™ and H#.
Versa-Rack allows you to count standard vials, miniature vials or Bio-Vials™ on the same
system. H# uses an external quench monitor (
137
Cs) for Automatic Quench Compensation
(AQC).
Enhancement Packs
Productivity Pack
This is an option for the Value System and includes Xtalscint
®
CPM/DPM, single- and
dual-label DPM and an RS-232 interface.
- LS 6000 Series 1
- Table of Contents 3
- Introduction 11
- Principles of Operation 12
- Operating the Instrument 12
- Operating Modes 13
- 1.3 Specifications 14
- Precautions and Limitations 15
- Figure 1.1 Standard Vial 15
- Miniature Vials 16
- 1.5 Hazards 17
- Maintenance 18
- Getting Started 19
- 2.2 Power Failure 21
- 2.3 Operating Controls 21
- Operator Control Key pad 22
- Using the Operating Controls 24
- 2.4 Using the Racks 25
- Using the Racks 26
- Setting Up the Racks 27
- 2.5 Preparing the Printer 29
- 2.6 System Setup 30
- Energy Scale 31
- Printer Paging 32
- Allow Interrupt Count 32
- System Setup 33
- Figure 2.9 RS232 Setup Menu 33
- Date/Time 35
- Auto DPM 35
- 2.7 Calibration 36
- Counting Samples 39
- Starting the Automatic Count 40
- Conducting Count Single Rack 41
- 3.4 Auto DPM 46
- 3.5 Multi-Task 47
- Interrupt Count in Multi-Task 48
- Multi-Task 50
- Interrupt Data 51
- Setting Up User Programs 59
- 4.2 Editing A User Program 61
- Editing A User Program 62
- Comments 65
- Counting Time 65
- Liquid or Xtal Scintillator 65
- Isotope 1 65
- Isotope 2 and Isotope 3 66
- Edit Other Parameters 67
- Counting Precision 69
- Background/Blank Subtraction 69
- Lum-Ex Correction 71
- Phase Monitor 72
- Low Level 72
- Low Count Reject 72
- Output Formats 72
- Count Data 75
- Raw Data 75
- Collecting Spectral Data 76
- 4.3 Copy User Program 77
- Copy User Program 78
- 5.2 CPM/Xtal CPM 80
- CPM/Xtal CPM 81
- Results for CPM/Xtal CPM 83
- 5.4 Auto DPM 85
- Setting Up Auto DPM 86
- Loading Samples for Auto DPM 86
- Results for Auto DPM 86
- Setting Up DPM 87
- 5.6 Xtal DPM 89
- Xtal DPM 90
- Setting Up Xtal DPM 91
- Loading Samples for Xtal DPM 92
- 5.7 DPM % of Reference 93
- Setting Up DPM % of Reference 94
- Xtal SL DPM % of Reference 95
- 5.9 Single Photon Monitoring 98
- Data Calculation 100
- Single Photon Monitoring 100
- Isotope/DPM Libraries 103
- Accessing the Isotope Library 104
- Printing the Isotope Library 104
- The Isotope Library 105
- Setup Menu 106
- PN 247971-F 108
- 5 minutes 109
- 6.2 Setting Up A Quench Curve 110
- Setting Up A Quench Curve 112
- Counting the Standards 114
- Editing Quench Curves 120
- Deleting A Quench Curve 124
- Manual Entry of Quench Curves 125
- 7.1 Chemiluminescence 127
- Interpretation of Results 128
- Reducing Single Photon Events 128
- 7.3 Two Phase Samples 129
- Procedures 131
- Analysis of Data 131
- H Counting Efficiency 132
- C Counting Efficiency 132
- Background 133
- Contamination 133
- Instrument Specifications 135
- Temperature Control Accessory 138
- Installation Requirements 139
- Electrical Requirements 140
- 0.5 to 1.0 m in 140
- Environment Requirements 142
- . Note that the output 144
- Datum IDs 146
- CHAPTER 1, PART 31 167
- Region I 170
- Region II 170
- Region III 170
- Region IV 170
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